Podium 1: Skeletal Disorders, Treatments & Rehabilitation

Murtaza Wasi, Tiankuo Chu, Rosa Guerra, Lidan You (U Toronto), Liyun Wang

University of Delaware

Chemotherapeutic agents such as anthracyclines directly affect bone cells, marrow and induce bone loss. Rats receiving anthracycline showed reduction in the densities of osteocytes and bone lining cells. Using a piezo1 channel agonist, Yoda1, with loading improved bone properties and bone formation in mice without and with breast cancer. Hence the aim of our study was to understand the efficacy of Yoda1 augmented exercise in mitigating bone loss after chemotherapy. Healthy aged mice were injected with Doxorubicin at two different doses: 2.5 and 5 mg/kg in 6 cycles. After one week of recovery time, two weeks of Yoda1 intraperitoneal injection and tibial loading (4.5 N peak load, 4 Hz, 300 cycles for 5 days/week) was performed. Cortical bone properties were analyzed from the micro CT data at weeks 0, 2 and 5 at two locations above Tibia-Femur Junction. Both doses of doxorubicin declined bone properties examined in our study with the higher dose causing drastic decline at both cortical regions, due to elevated bone resorption. Loading alone did not have any beneficial effect on the cortex. Yoda1 treatment dampened the decline in the cortical polar moment of inertia (Ct.pMOI). The Yoda1 augmented loading group resulted in significant improvement in Ct.pMOI.

Research Area: Bone