Poster 1: Cell and Tissue Studies

Tiankuo Chu, Jason Jiang, Wiley Gong, Murtaza Wasi, Rosa Guerra, Shubo Wang, Lidan You (University of Toronto), Liyun Wang*

University of Delaware

Physical exercise, as an adjuvant therapeutic, improves bone quality, but routine aerobic or strength exercise can be challenging for some elderly cancer patients with unintended injuries.1?2 Even though whole-body vibration (WBV) is safer and easier-to-perform and shows overall wellness benefits for metastatic patients including bone protection, but the bone remodeling respond is limited due to the aging effect.3 What’s more, radiotherapy is a common treatment of cancer inhibition and pain management, but radiation was found to increase the apoptosis of osteocytes (the major mechanosensing cells in bone).4 Yoda1, an agonist to the mechanosensitive Piezo1 channels highly expressed in osteocytes, promotes bone growth in young mice,5 but its effects on mature bone in the presence of WBV and radiotherapy remain to be determined. In our study, the 8-month-old mature Balb/c and C57BL/6J female mice received WBV (12 Hz, 0.25 g, 30 min with 7.5 min rest in between) 1h after Yoda1 injection, 5 days/week for 4 weeks after 8Gy radiation twice before intervention. Under the challenges of radiation, we did observe smaller pMOI decline (a trend) in the Yoda1-augmented WBV group and a significant increase in bone formation at week 2 for the combined treatment. Besides, relative to non-treated groups, the Yoda1-augmented WBV significantly inhibited the osteoclast’s TRAP activity regardless of radiation. Our results demonstrated the benefits of Yoda1-augmented WBV are retained in mature mice after radiotherapy.
REFERENCES: [1]. Chen+ 2017. [2]. Alfano+ 2012, [3]. Margaret+ 2021, [4]. Abhishek+ 2017, [5]. Li+ 2019

Research Area: Bone